富山大学 学術研究部医学系 免疫学

研究業績

  1. Kimura M, Egawa K, Ozawa T, Kishi H, Shimojima M, Taniguchi S, Fukushi S, Fujii H, Yamada H, Tan L, Sano K, Katano H, Suzuki T, Morikawa S, Saijo M, Tani H. Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein. Virology, 556: 124-132, 2021.
  2. Tani H, Kimura M, Tan L, Yoshida Y, Ozawa T, Kishi H, Fukushi S, Saijo M, Sano K, Suzuki T, Kawasuji H, Ueno A, Miyajima Y, Fukui Y, Sakamaki I, Yamamoto Y, Morinaga Y. Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein. Virol. J., 18: 16, 2021.
  3. Ozawa T, Kobayashi E, Hamana H, Nakamura T, Lyu F, Hayashi A, Muraguchi A, Kishi H. Rapid and efficient generation of T-cell receptor-like antibodies using chip-based single-cell analysis. Eur. J. Immunol., in press, 2021.
  4. Tsuda S, Nakashima A, Morita K, Shima T, Yoneda S, Kishi H, Saito S. The role of decidual regulatory T cells in the induction and maintenance of fetal antigen-specific tolerance: Imbalance between regulatory and cytotoxic T cells in pregnancy complications. Hum. Immunol., in press, 2021.

  1. Hamamoto Y, Ouhara K, Munenaga S, Shoji M, Ozawa T, Hisatsune J, Kado I, Kajiya M, Matsuda S, Kawai T, Mizuno N, Fujita T, Hirata S, Tanimoto K, Nakayama K, Kishi H, Sugiyama E, Kurihara H. Effect of porphyromonas gingivalis infection on gut dysbiosis and resultant arthritis exacerbation in mouse model. Arthritis Res. Ther. 22: 249, 2020.
  2. Sakai T, Terakura S, Miyao K, Okuno S, Adachi Y, Umemura K, Julamanee J, Watanabe K, Hamana H, Kishi H, Leitner J, Steinberger P, Nishida T, Murata M, Kiyoi H. Artificial T cell adaptor molecule-transduced TCR-T cells demonstrated improved proliferation only when transduced in a higher intensity. Mol. Ther. Oncolytics, 18: 613-622, 2020.
  3. Mou Z, Barazandeh A, Hamana H, Kishi H, Zhang X, Jia P, Ikeogu N, Onyilagha C, Gupta G, Uzonna J. Identification of a protective Leishmania antigen dihydrolipoyl dehydrogenase and its responding CD4+ T cells at clonal level. J. Immunol, 205(5): 1355-1364, 2020.
  4. Ozawa T, Ouhara K, Tsuda R, Munenaga S, Kurihara H, Kohno H, Hamana H, Kobayashi E, Taki H, Tobe K, Sugiyama E, Muraguchi A, Kishi H. Physiological target, molecular evolution and pathogenic functions of a monoclonal ACPA obtained from an RA patient. Arthritis & Rheumatology, 72(12): 2040-2049, 2020.
  5. Iwasaki K, Hamana H, Kishi H, Yamamoto T, Hiramitsu T, Okada M, Tomosugi T, Takeda A, Narumi S, Watarai Y, Miwa Y, Okumura M, Matsuoka Y, Horimi K, Muraguchi A, Kobayashi T. The suppressive effect on CD4 T-cell alloresponse against endothelial HLA-DR via PD-L1 induced by anti-A/B ligation. Clin. Exp. Immunol., 202: 249-261, 2020.
  6. Mizumaki H, Hosomichi K, Hosokawa K, YoroidakaT, Imi T, Zaimoku Y, Katagiri T, Nguyen MAT, Tran DC, Elbadry MIY, Chonabayashi K, Yoshida Y, Takamatsu H, Ozawa T, Azuma F, Kishi H, Fujii Y, Ogawa S, Tajima A, Nakao S. A frequent nonsense mutation in exon 1 across certain HLA-A and -B alleles in leukocytes of patients with acquired aplastic anemia. Haematologica, in press, 2020.
  7. Morita K, Tsuda S, Kobayashi E, Hamana H, Tsuda K, Shima T, Nakashima A, Ushijima A, Kishi H, Saito S. Analysis of TCR repertoire and PD-1 expression in decidual and peripheral CD8+ T cells reveals distinct immune mechanisms in miscarriage and preeclampsia. Front Immunol., 11: 1082, 2020.
  8. Sukegawa K, Shitaoka K, Hamana H, Kobayashi E, Miyahara Y, Fujii K, Tsuda K, Saeki S, Nagata T, Ozawa T, Saito S, Fujii T, Muraguchi A, Shiku H, Kishi H. T cell receptor (TCR) repertoire analysis of tumor-infiltrating lymphocytes of primary and metastatic tumors in colorectal cancer shows the relationship between TCR clonotype and PD-1 expression. Eur. J. Immunol., 50: 1580-1590, 2020.
  9. Miyoshi S, Tokunaga S, Ozawa T, Takeda H, Aono M, Miyoshi T, Kishi H, Muraguchi A, Shimizu S, Nozawa A, Sawasaki T. Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses. PloS One, 15(4): e0229196, 2020.
  10. Hiramoto T, Tahara M, Liao J, Soda Y, Miura Y, Kurita R, Hamana H, Inoue K, Kohara H, Miyamoto S, Hijikata Y, Okano S, Yamaguchi Y, Oda Y, Ichiyanagi K, Toh H, Sasaki H, Kishi H, Ryo A, Muraguchi A, Takeda M, Tani K. Non-transmissible MV vector with segmented RNA genome establishes different types of iPSCs from hematopoietic cells. Mol. Ther., 28(1): 129-141, 2020.

  1. Iiizumi S, Ohtake J, Murakami N, Kouro T, Kawahara M, Isoda F, Hamana H, Kishi H, Nakamura N, Sasada T. Identification of novel HLA Class Ⅱ-restricted neoantigens derived from driver mutations. Cancers, 11(2): E266, 2019.
  2. Kishi H, Ozawa T, Hamana H, Kobayashi E, Muraguchi A. Isolation of antigen-specific, antibody-secreting cells using a chip-based immunospot array. Methods Mol. Biol., 1904: 147-162, 2019.
  3. Lyu F, Ozawa T, Hamana H, Kobayashi E, Muraguchi A, Kishi H. A novel and simple method to produce large amounts of recombinant soluble peptide/major histocompatibility complex monomers for analysis of antigen-specific human T cell receptors. New Biotechnology, 49: 169-177, 2019.

  1. Yamada H, Ozawa T, Kishi H, Okada S, Nakashima Y, Muraguchi A, Yoshikai Y. Cutting Edge: B Cells Expressing Cyclic Citrullinated Peptide-Specific Antigen Receptor Are Tolerized in Normal Conditions. J Immunol., 201(12): 3492-3496, 2018.
  2. Tsuda S, Zhang X, Hamana H, Shima T, Ushijima A, Tsuda K, Muraguchi A, Kishi H, Saito S. Clonally Expanded Decidual Effector Regulatory T Cells Increase in Late Gestation of Normal Pregnancy, but Not in Preeclampsia, in Humans. Front Immunol., 9: 1934, 2018.
  3. Dai T, Hu Y, Lv F, Ozawa T, Sun X, Huang J, Han X, Kishi H, Muraguchi A, Jin A. Analysis of the clinical significance of DCLK1+ colorectal cancer using novel monoclonal antibodies against DCLK1. OncoTargets Ther., 11: 5047-5057, 2018.
  4. Hu Z, Anandappa AJ, Sun J, Kim J, Leet DE, Bozym DJ, Chen C, Williams L, Shukla SA, Zhang W, Tabbaa D, Steelman S, Olive O, Livak KJ, Kishi H, Muraguchi A, Guleria I, Stevens J, Lane WJ, Burkhardt UE, Fritsch EF, Neuberg D, Ott PA, Keskin DB, Hacohen N, Wu CJ. A cloning and expression system to probe T-cell receptor specificity and assess functional avidity to neoantigens. Blood, 132(18): 1911-1921, 2018.
  5. Okumura M, Ozawa T, Hamana H, Norimatsu Y, Tsuda R, Kobayashi E, Shinoda K, Taki H, Tobe K, Imura J, Sugiyama E, Kishi H, Muraguchi A. Autoantibodies reactive to PEP08 are clinically related with morbidity and severity of interstitial lung disease in connective tissue diseases. Eur. J. Immunol., 48(10): 1717-1727, 2018.
  6. Tsuruta M., Ueda S., Yew PY., Fukuda I., Yoshimura S., Kishi H., Hamana H., Hirayama M., Yatsuda J., Irie A., Senju S., Yuba E., Kamba T., Eto M., Nakayama H., Nishimura Y.: Bladder cancer-associated cancer-testis antigen-derived long peptides encompassing both CTL and promiscuous HLA class Ⅱ-restricted Th cell epitopes induced CD4+ T cells expressing converged T-cell receptor genes in vitro. Oncoimmunology, 7(4): e1415687, 2018.
  7. Tanaka T., Zhou Y., Ozawa T., Okizono R., Banba A., Yamamura T., Oga E., Muraguchi A., Sakurai H.: Ligand-activated epidermal growth factor receptor (EGFR) signaling governs endocytic trafficking of unliganded receptor monomers by non-canonical phosphorylation. J. Biol. Chem., 293(7): 2288-2301, 2018.
  8. Espinoza JL., Elbadry MI., Chonabayashi K., Yoshida Y., Katagiri T., Harada K., Nakagawa N., Zaimoku Y., Imi T., Hassanein HA., Khalifa A. Noreldin A., Takenaka K., Akashi K., Hamana H., Kishi H., Akatsuka Y., Nakao S.: Hematopoiesis by iPSC-derived hematopoietic stem cells of aplastic anemia that escape cytotoxic T-cell attack. Blood Advances, 2(4): 390-400, 2018.
  9. Shitaoka K., Hamana H., Kishi H., Hayakawa Y., Kobayashi E., Sukegawa K., Piao X., Lyu F., Nagata T., Sugiyama D., Nishikawa H., Tanemura A., Katayama I., Murahashi M., Takamatsu Y., Tani K., Ozawa T., Muraguchi A.: Identification of tumoricidal TCRs from tumor-infiltrating lymphocytes by single-cell analysis. Cancer Immunol. Res., 6(4): 378-388, 2018.
  10. Ozawa T., Masaki H., Takasaki T., Aoyama I., Yumisashi T., Yamanaka A., Konishi E., Ohnuki Y., Muraguchi A., Kishi H.: Human monoclonal antibodies against West Nile virus from Japanese encephalitis-vaccinated volunteers. Antiviral Res., 154: 58-65, 2018.
  11. Tanaka T., Ozawa T., Oga E., Muraguchi A., Sakurai H.: Cisplatin-induced non-canonical endocytosis of EGFR via p38 phosphorylation of the C-terminal region containing Ser-1015 in non-small cell lung cancer cells. Oncology Lett., 15: 9251-9256, 2018.
  12. Miyao K., Terakura S., Okuno S., Julamanee J., Watanabe K., Hamana H., Kishi H., Sakemura R., Koyama D., Goto T., Nishida T., Murata M., Kiyoi H.: Introduction of genetically modified CD3ζ improves proliferation and persistence of antigen-specific CTLs. Cancer Immunol. Res., 6(6): 733-744, 2018.

  1. Nakagawa H., Mizukoshi E., Kobayashi E., Tamai T., Hamana H., Ozawa T., Kishi H., Kitahara M., Yamashita T., Arai K., Terashima T., Iida N., Fushimi K., Muraguchi A., Kaneko S.: Association between high-avidity T-cell receptors, induced by Alpha Fetoprotein-derived peptides, and anti-tumor effects in patients with hepatocellular carcinoma. Gastroenterology, 152(6): 1395-1406, 2017.
  2. Miyama T., Kawase T., Kitaura K., Chishaki R., Shibata M., Oshima K., Hamana H., Kishi H., Muraguchi A., Kuzushima K., Saji H., Shin-I T., Suzuki R., Ichinohe T.: Highly functional T-cell receptor repertoires are abundant in stem memory t cells and highly shared among individuals. Sci. Rep., 7(1): 3663, 2017.
  3. Zaimoku Y., Takamatsu H., Hosomichi K., Ozawa T., Nakagawa N., Imi T., Maruyama H., Katagiri T., Kishi H., Tajima A., Muraguchi A., Kashiwase K., Nakao S.: Identification of an HLA class Ⅰ allele closely involved in the auto-antigen presentation in acquired aplastic anemia. Blood, 129(21): 2908-2916, 2017.
  4. Guo L., Sun X., Hao Z., Huang J., Han X., You Y., Li Y., Shen M., Ozawa T., Kishi H., Muraguchi A., Jin A.: Identification of novel epitopes with agonistic activity for the development of tumor immunotherapy targeting TRAIL-R1. Journal of Cancer, 8(13): 2542-2553, 2017.
  5. Lo CY., Strobl SL., Dunham K., Wang W., Stewart L., Misplon JA., Garcia M., Gao J., Ozawa T., Price GE., Navidad J., Gradus S., Bhattacharyya S., Viboud C., Eichelberger MC., Weiss CD., Gorski J., Epstein SL.: Surveillance study of Influenza occurrence and immunity in a Wisconsin cohort during the 2009 pandemic. Open Forum Infect. Dis., 4(2): ofx023, 2017.
  6. Feng Y., Brazin KN., Kobayashi E., Mallis RJ., Reinhertz EL., Lang MJ.: Mechanosensing drives acuity of αβ T-cell recognition. Proc. Natl. Acad. Sci. USA., 114(39): E8204-E8213, 2017.

  1. Piao X., Ozawa T., Hamana H., Shitaoka K., Jin A., Kishi H., Muraguchi A.: TRAIL-receptor 1 IgM antibodies strongly induce apoptosis in human cancer cells in vitro and in vivo. Oncoimmunology, 5(5): e1131380, 2016.
  2. Hamana H., Shitaoka K., Kishi H., Ozawa T., Muraguchi A.: A novel, rapid and efficient method of cloning functional antigen-specific T-cell receptors from single human and mouse T-cells. Biochem. Biophys. Res. Commun., 474: 709-714, 2016.
  3. Hao Z., Han X., Sun X., Shen M., Huang J., Li Y., Ozawa T., Pang D., Jin S., Kishi H., Muraguchi A., Jin A.: Fully human monoclonal antibodies to TRAIL-R1 enhance TRAIL-induced apoptosis via activation of caspase-8 pathway. Biochem. Biophys. Res. Commun., 475: 238-244, 2016.
  4. Nagatoshi Y., Ikeda M., Kishi H., Hiratsu K., Muraguchi A., Ohme-Takagi M.: Induction of a dwarf phenotype with IBH1 may enable increased production of plant-made pharmaceuticals in plant factory conditions. Plant Biotechnol J. 14: 887-894, 2016.
  5. Ohnaga T., Shimada Y., Takata K., Obata T., Okumura T., Nagata T., Kishi H., Muraguchi A., Tsukada K.: Capture of esophageal and breast cancer cells with polymeric microfluidic devices for CTC isolation. Molecular and Clinical Oncology, 4: 599-602, 2016.
  6. Kawasaki Y, Sakimura A, Park CM, Tomaru R, Tanaka T, Ozawa T, Zhou Y, Narita K, Kishi H, Muraguchi A, Sakurai H. Feedback control of ErbB2 via ERK-mediated phosphorylation of a conserved threonine in the juxtamembrane domain. Sci Rep. 2016 Aug 17; 6: 31502.

  1. Hamada-Tsutsumi S., Iio E., Watanabe T, Murakami S., Isogawa M., Iijima S., Inoue T., Matsunami K., Tajiri K., Ozawa T., Kishi H., Muraguchi A., Joh T., Tanaka Y.: Validation of cross-genotype neutralization by hepatitis B virus-specific monoclonal antibodies by in vitro and in vivo infection. PLoS One, 10(2): e0118062, 2015.
  2. Simhadri VR., Dimitrova M., Mariano JL., Zenarruzabeitia O., Zhong W., Ozawa T., Muraguchi A., Kishi H., Eichelberger MC., Borrego F.A.: Human Anti-M2 Antibody Mediates Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Cytokine Secretion by Resting and Cytokine-Preactivated Natural Killer (NK) Cells. PLoS One, 10(4): e0124677, 2015.
  3. Tsuda R., Ozawa T., Kobayashi E., Hamana H., Taki H., Tobe K., Sugiyama E., Iwamoto M., Imura J., Kishi H., Muraguchi A.: Monoclonal antibody against citrullinated peptides obtained from rheumatoid arthritis patients reacts with numerous citrullinated microbial and food proteins. Arthritis Rheumatol., 67(8): 2020-31, 2015.
  4. Takeda H., Ogasawara T., Ozawa T., Muraguchi A., Jih PJ., Morishita R., Uchigashima M., Watanabe M., Fujimoto T., Iwasaki T., Endo Y., Sawasaki T.: Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay. Sci Rep., 5: 11333, 2015.
  5. Mizukoshi E., Nakagawa H., Kitahara M., Yamashita T., Arai K., Sunagozaka H., Fushimi K., Kobayashi E., Kishi H., Muraguchi A., Kaneko S.: Immunological features of T cells induced by human telomerase reverse transcriptase-derived peptides in patients with hepatocellular carcinoma. Cancer Lett., 364(2): 98-105, 2015.
  6. Mou Z., Li J., Boussoffara T., Kishi H., Hamana H., Ezzati P., Hu C., Yi W., Liu D., Khadem F., Okwor I., Jia P., Shitaoka K., Wang S., Ndao M., Petersen C., Chen J., Rafati S., Louzir H., Muraguchi A., Wilkins JA., Uzonna JE.: Identification of broadly conserved cross-species protective Leishmania antigen and its responding CD4+ T cells. Sci. Transl. Med., 7(310): 310ra167, 2015.

  1. Kobayashi E., Kishi H., and Muraguchi A.: A novel system for cloning human TCRs: Cutting short the way to TCR-based anticancer therapy. OncoImmunology, 3: e27258, 2014.
  2. Kobayashi E., Kishi H., Ozawa T., Horii M., Hamana H., Nagai T., and Muraguchi A.: Retroviral vectors for homologous recombination provide efficient cloning and expression in mammalian cells. Biochem. Biophys. Res. Commun., 444: 319-324, 2014.
  3. Kobayashi E., Kishi H., Ozawa T., Hamana H., Nakagawa H., Jin A., Lin Z., and Muraguchi A.: A chimeric antigen receptor for TRAIL-receptor 1 induces apoptosis in various types of tumor cells. Biochem. Biophys. Res. Commun., 453: 798-803, 2014.
  4. Honda H., Nagai Y., Matsunaga T., Okamoto N., Watanabe Y., Tsuneyama K., Hayashi H., Fujii I., Ikutani M., Hirai Y., Muraguchi A., and Takatsu.: Isoliquiritigenin is a potent inhibitor of NLRP3 inflammasome activation and diet-induced adipose tissue inflammation. J. Leukocyte Biol., 96: 1087- , 2014.

  1. Ohnaga T., Shimada Y., Moriyama M., Kishi H., Obata T., Takata K., Okumura T., Nagata T., Muraguchi A., and Tsukada K.: Polymeric microfluidic devices exhibiting sufficient capture of cancer cell line for isolation of circulating tumor cells. Biomed Microdevices, 15: 611-616, 2013.
  2. Kurosawa N., Fujimoto R., Ozawa T., Itoyama T., Sadamori N., and Isobe M.: Reduced level of the BCL11B protein is associated with adult T-cell leukemia/lymphoma. PLoS One. 8: e55147, 2013.
  3. Ikeda M., Tsuno S., Sugiyama T., Hashimoto A., Yamoto K., Takeuchi K., Kishi H., Mizuguchi H., Kohsaka SI., Yoshioka T.: Ca2+ spiking activity caused by the activation of store-operated Ca2+ channels mediates TNF-α release from microglial cells under chronic purinergic stimulation. Biochim Biophys Acta. 1833: 2573-2585, 2013.
  4. Suzuki M., Yamanoi A., Machino Y., Kobayashi E., Fukuchi K., Tsukimoto M., Kojima S., Kohroki J., Akimoto K., and Masuho Y.: Cleavage of the interchain disulfide bonds in rituximab increases its affinity for FcγRIIIA. Biochem Biophys Res Commun., 436: 519-24, 2013.
  5. Kobayashi E., Mizukoshi E., Kishi H., Ozawa T., Hamana H., Nagai T., Nakagawa H., Jin A., Kaneko S., and Muraguchi A.: A novel cloning and expression system yields and validates TCRs from blood lymphocytes of cancer patients within 10 days. Nature Medicine, 19: 1542-1546, 2013.

  1. Ozawa T., Piao X., Kobayashi E., Zhou Y., Sakurai H., Andoh T., Jin A., Kishi H., and Muraguchi A.: A novel rabbit immunospot array assay on a chip allows for the rapid generation of rabbit monoclonal antibodies with high affinity. PLoS One, 7: e52383, 2012.
  2. Ozawa T., Horii M., Kobayashi E., Jin A., Kishi H., and Muraguchi A.: The binding affinity of a soluble TCR-Fc fusion protein is significantly improved by crosslinkage with an anti-Cβantibody. Biochem. Biophys. Res. Commun., 422: 245-249, 2012.
  3. Sun X., Saito M., Sato Y., Chikata T., Naruto T., Ozawa T., Kobayashi E., Kishi H., Muraguchi A., and Takiguchi M.: Unbiased analysis of TCRα/β chains at the single-cell level in human CD8+ T-cell subsets. PLoS One, 7: e40386, 2012.
  4. Honda H., Nagai Y., Matsunaga T., Saitoh S., Akashi-Takamura S., Hayashi H., Fujii I., Miyake K., Muraguchi A., and Takatsu K.: Glycyrrhizin and isoliquiritigenin suppress the LPS sensor Toll-like receptor 4/MD-2 complex signaling in a different manner. Journal of Leukocyte Biology, 91: 967-976, 2012.
  5. Fujimoto R., Ozawa T., Itoyama T., Sadamori N., Kurosawa N., and Isobe M.: HELIOS-BCL11B fusion gene involvement in a t(2;14)(q34;q32) in an adult T-cell leukemia patient. Cancer Genetics, 205: 356-364, 2012.

  1. Jin A., Ozawa T., Tajiri K., Obata T., Kishi H., and Muraguchi A.: Rapid isolation of antigen-specific antibody-secreting cells using a chip-based immunospot array. Nat. Protoc., 6: 668-676, 2011.
  2. Ozawa T., Jin A., Tajiri K., Takemoto M., Okuda T., Shiraki K., Kishi H., and Muraguchi A.: Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus. Antiviral Res., 91: 283-287, 2011.
  3. Takano S., Takahashi Y., Kishi H., Taguchi Y., Takashima S., Tanaka K., Muraguchi A., and Mori H.: detection of autoantibody against extracellular epitopes of N-methyl-D-aspartate receptor by cell-based assay. Neurosci. Res., 71: 294-302, 2011.
  4. Tomohiro T., Kato K., Masuda S., Kishi H., and Hatanaka Y. : Photochemical construction of coumarin fluorophore on affinity-anchored protein. Bioconjug. Chem., 22: 315-318, 2011.
  5. Matsushita H., Endo S., Kobayashi E., Sakamoto Y., Kobayashi K., Kitaguchi K., Kuroki K., Söderhäll A., Maenaka K., Nakamura A., Strittmatter SM., and Takai T. : Differential but competitive binding of Nogo protein and class I major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells. J. Biol. Chem., 286: 25739-25747, 2011.

  1. Tajiri K., Ozawa T., Jin A.-S., Tokimitsu Y., Minemura M., Kishi H., Sugiyama T., Muraguchi A.: Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine. Antiviral. Res., 87: 40-49, 2010.
  2. Lin Z.-Z., Jin A.-S., Ozawa T., Tajiri K., Obata T., Ishida I., Jin F., Kishi H., Muraguchi A.: Post-translational modification of TRAIL receptor type 1 on various tumor cells and the susceptibility of tumors to TRAIL-induced apoptosis. Biochem. Biophys. Res. Commun., 395: 251-257, 2010.
  3. Kondo S., Kishi H., Muraguchi A.: Regulatory role of leukocyte common antigen-related molecule (LAR) in thymocyte differentiation. Eur. J. Immunol., 40: 1296-1302, 2010.
  4. Jin A., Ozawa T., Tajiri K., Lin Z., Obata T., Ishida I., Kishi H., and Muraguchi A.: Generation of TRAIL-receptor 1-specific human monoclonal Ab by a combination of immunospot array assay on a chip and human Ab-producing mice. Eur. J. Immunol., 40: 3591-3593, 2010.

  1. Jin A.-S., Ozawa T., Tajiri K., Obata T., Kondo S., Kinoshita K., Kadowaki S., Takahashi K., Kishi H., Sugiyama T., Muraguchi A.: A rapid and efficient single cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood. Nat. Med., 15: 1088-1092, 2009.
  2. Kinoshita K., Ozawa T., Tajiri K., Kadowaki S., Kishi H., Muraguchi A.: Identification of antigen-specific B cells by concurrent monitoring of intracellular Ca2+ mobilization and antigen binding with microwell array chip system equipped with a CCD imager. Cytometry Part A. 75A: 682-687, 2009.
  3. Tajiri K., Kishi H., Ozawa T., Sugiyama T., Muraguchi A.: SFMAC: A novel method for analyzing multiple parameters on lymphocytes with a single fluorophore in cell-microarray system. Cytometry Part A. 75A: 282-288 2009.
  4. Motoki K., Kishi H., Hori E., Tajiri K., Nishijo H., Muraguchi A.: The direct excitatory effect of IL-1β on cerebellar Purkinje cell. Biochem. Biophys. Res. Commun., 379: 665-668, 2009.
  5. Ozawa T., Kinoshita K., Kadowaki S., Tajiri K., Kondo S., Honda R., Ikemoto M., Piao L.-X., Morisato A., Fukurotani K., Kishi H., Muraguchi A.: MAC-CCD system: a novel lymphocyte microwell-array chip system equipped with CCD scanner to generate human monoclonal antibodies against influenza virus. Lab on a Chip, 9: 158-163, 2009.

  1. Andoh T., Kishi H., Motoki K., Nakanishi K., Kuraishi Y., Muraguchi A.: Protective effect of IL-18 on kainate- and IL-1β-induced cerebellar ataxia in mice. J. Immunol., 180: 2322-2328, 2008.
  2. Ozawa T., Tajiri K., Kishi H., Muraguchi A.: Comprehensive analysis of the functional TCR repertoire at the single-cell level. Biochem. Biophys. Res. Commun., 367: 820-825, 2008.

  1. Tokimitsu Y., Kishi H., Kondo S., Honda R., Tajiri K., Motoki K., Ozawa T., Kadowaki S., Obata T., Fujiki S., Tateno C., Takaishi H., Chayama K., Yoshizato K., Tamiya E., Sugiyama T., Muraguchi A.: Single lymphocyte analysis with a microwell array chip. Cytometry Part A, 71A: 1003-1010, 2007.
  2. Tajiri K., Kishi H., Tokimitsu Y., Kondo S., Ozawa T., Kinoshita K., Jin A., Kadowaki S., Sugiyama T., Muraguchi A.: Cell-microarray analysis of antigen-specific B-cells: single cell analysis of antigen receptor expression and specificity. Cytometry Part A, 71A: 961-967, 2007.
  3. Shoji M., Kondo T., Honoki H., Nakajima T., Muraguchi A., Saito M.: Investigation of monitoring for internal exposure by urine bioassay in a biomedical research facility. Radiat. Prot. Dosimetry, 127: 456-460, 2007.

  1. Wang H. –J., Yu C. - L., Kishi H., Motoki M., Muraguchi A.: Suppression of experimental osteoarthritis by adenovirus-mediated double gene transfer. Chin. Med. J., 119: 1365-1373, 2006.
  2. Hashioka S., Obata T., Tokimitsu Y., Fujiki S., Nakazato H., Murguchi A., Kishi H., Tanino K.: Trapping and collection of lymphocytes using microspot array chip and magnetic beads. Japanese Journal of Applied Physics, 45: 2850-2853, 2006.
  3. Ozawa T., Kishi H., Muraguchi A.: Amplification and analysis of cDNA generated from a single cell by 5’-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells. Bio Techniques, 40: 469-478, 2006.

  1. Shoji M., Kondo T., Honoki H., Nakajima T., Muraguchi A., and Saito M.: A case study of the estimation of occupational internal dose using urinary excretion data obtained in a biomedical research facility. Health Phys., 89: 618-627, 2005.
  2. Yamamura S., Kishi H., Tokimitsu Y., Kondo S., Honda R., Rao S.R., Omori M., Tamiya E., and Muraguchi A.: Single-cell microarray for analyzing cellular response. Anal. Chem., 77: 8050-8056, 2005.
  3. Wei X. –C., Dohkan J., Kishi H., Wu C. –X., Kondo S., and Muraguchi A.: Characterization of the proximal enhancer element and transcriptional regulatory factors for murine recombination activating gene-2. Eur. J. Immunol., 35: 612-621, 2005.
  4. Shinoda C., Maruyama M., Fujishita T., Dohkan J., Oda H., Shinoda K., Yamada T., Miyabayashi K., Hayashi R., Kawagishi Y., Fujita T., Matsui S., Sugiyama E., Muraguchi A., and Kobayashi M.: Doxorubicin induces expression of multidrug resistance-associated protein 1 in human small cell lung cancer cell lines by the c-jun N-terminal kinase pathway. Int. J. Cancer, 117: 21-31, 2005.

  1. Yamamoto T., Imoto S., Sekine Y., Sugiyama K., Akimoto T., Muraguchi A., and Matsuda T.: Involvement of NF-κB in TGF-β-mediated suppression of IL-4 signaling. Biochem. Biophys. Res. Commun., 313: 627-634, 2004.
  2. Wu C. –X., Zhao W. –P., Kishi H., Dokan J., Jin Z. –X., Wei X. –C., Yokoyama K., and Muraguchi A.: Activation of mouse RAG-2 promoter by Myc-associated zinc finger protein. Biochem. Biophys. Res. Commun., 317: 1096-1102, 2004.
  3. Barnhill JC., Stokes AJ., Koblan-Huberson M., Shimoda LM., Muraguchi A., Adra CN., and Turner H.: RGA protein associates with a TRPV ion channel during biosynthesis and trafficking. J. Cell. Biochem., 91: 808-820, 2004.

  1. Tanaka K., Yamamoto T., Aikawa Y., Kizawa K., Muramoto K., Matsuno H., and Muraguchi A.: Inhibitory effects of an anti-rheumatic agent T-614 on immunoglobulin production by cultured B cells and rheumatoid synovial tissues engrafted into SCID mice. Rheumatology, 42: 1365-1371, 2003.
  2. Liu Q. -L., Kishi H., Ohtsuka K., and Muraguchi A.: Heat-shock protein 70 binds caspase-activated DNase and enhances its activity in TCR-stimulated T cells. Blood, 102: 1788-1796, 2003.
  3. Kondo S., Kishi H., Tokimitsu Y., and Muraguchi A.: Possible involvement of glial cell line-derived neurotrophic factor (GDNF) and its receptor, GFRα1, in survival and maturation of thymocytes. Eur. J. Immunol., 33: 2233-2240, 2003.
  4. Kawakami T., Nagata T., Muraguchi A., and Nishimura T.: Proteomic approach to apoptotic thymus maturation. J. Chromatography B., 787: 223-229, 2003.

  1. Jin Z. -X., Kishi H., Wei X. -C., Matsuda T., Saito S. and Muraguchi A.: LEF-1 binds and activates the RAG-2 promoter together with c-Myb and Pax-5 in immature B cells. J. Immunol. 169: 3783-3792, 2002.
  2. Wei X. - C., Kishi H., Jin Z. -X., Zhao W. -P., Kondo S., Matsuda T., Saito S. and Muraguchi A.: Characterization of chromatin structure and enhancer elements for murine recombination activating gene-2. J. Immunol. 169: 873-881, 2002.
  3. Nagata T., Kishi H., Liu Q. -L., Matsuda T., Imanaka T., Tsukada K., Kang D. and Muraguchi A.: The regulation of DNAse activities in subcellular compartments of activated thymocytes. Immunology. 105: 399-406, 2002.
  4. Kishi H., Jin Z. -X., Wei X. -C., Nagata T., Matsuda T., Saito S. and Muraguchi A.: Cooperative binding of c-Myb and Pax-5 activates the RAG-2 promoter in immature B cells. Blood. 99: 576-583, 2002.

  1. Matsuda T., Yamamoto T., Muraguchi A. and Saatcioglu F.: Cross-talk between transforming growth factor-β and estrogen receptor signaling through Smad3. J. Biol. Chem. 276: 42908-42914, 2001.
  2. Yoshino T., Kishi H., Nagata T., Tsukada K., Saito S. and Muraguchi A.: Differential involvement of p38 MAP kinase pathway and Bax translocation in the mitochondria-mediated cell death in TCR- and dexamethasone-stimulated thymocytes. Eur. J. Immunol. 31: 2702-2708, 2001.
  3. Matsuda T., Junicho A., Yamamoto T., Kishi H., Korkmaz K., Saatcioglu F., Fuse H. and Muraguchi A.: Cross-talk between signal transducer and activator of transcription 3 and androgen receptor signaling in prostate carcinoma cells. Biochem. Biophys. Res. Commun. 283: 179-187, 2001.
  4. Yamamoto T., Matsuda T., Muraguchi A., Miyazono K. and Kawabata M.: Cross-talk between IL-6 and TGF-β signaling in hepatoma cells. FEBS Lett. 492: 247-253, 2001.
  5. Yamamoto T., Matsuda T., Junicho A., Kishi H., Yoshimura A. and Muraguchi A.: Hematopoietic cell-specific adapter proteins, SLP-76 and BLNK, effectively activate NF-AT as well as NF-κB by Syk and Tec PTKs in non-lymphoid cell lines. FEBS Lett. 491: 272-278, 2001.